External factors
| External factors: |
ABT-737 |
| Category: |
Chemical compounds |
| Phenotype: |
Prostate cancer |
| Experimental category: |
HL |
| Experiment: |
MTT assay//BrdU assay//Colony formation assay |
| Description: |
MTT assay: Long-term exposure of PV-10 cells to ABT-737 induced significant growth inhibition and a G1/S cell cycle blockade. Brdu assay//Colony formation assay: a dose-dependent inhibition of colony formation and an inhibition of BrdU incorporation was seen after the addition of ABT-737 to both PV-10 and 22Rv1 cells. |
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Regulatory relationship
| R-EF-Target gene:
|
Activation |
| Target gene experiment: |
Western blot//SA-β-gal activity assay |
| Target gene description: |
Western blot: In a pool of PV-10 cells ATM levels were decreased by shRNA, we found that a marked reduction in ATM levels prevented ABT-737 from inducing γ-H2AX.SA-β-gal activity assay: ABT-737 treatment of cells with lower levels of ATM protein kinase caused a markedly reduced level of SA-β-Gal and senescence-like morphologic changes .we measured levels of IL-6 and IL-8 mRNA in PV-10-shRNAmir-ATM and PV-10-shRNAmir-control cells. QT-PCR analysis showed that ATM knockdown prevented the ABT-737–mediated induction of IL-6 and IL-8 mRNA. |
| Regulatory pathway:
|
p53-p21 |
| Official symbol(s): |
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TP53-CDKN1A
|
| Pathway experiment: |
Western blot//SA-β-gal activity assay |
| Pathway description: |
Western blot: Both PV-10 and 22Rv1 cells when treated with ABT-737 show increased levels of both wild-type p53 and p21 protein levels. We find that ABT-737 treatment inhibits Cdk2 activity in a dose-dependent fashion in both PV-10 and 22Rv1 cells. Western blot//SA-β-gal activity assay: Western blot analysis confirmed that after ABT-737 treatment overexpression of DN p53 caused a substantial reduction in p21 and inhibited the ability of ABT-737 to induce SA-β-Gal. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.