External factors
| External factors: |
GSK461364 |
| Category: |
Chemical compounds |
| Experimental category: |
HL |
| Cell name: |
U2OS,MG-63,SJSA |
| Experiment: |
Flow cytometry//MTT assay//Trypan blue staining//SA-β-gal activity assay//qRT-PCR |
| Description: |
Flow cytometric analysis of DNA content in cells treated with GSK461364 for 24 h demonstrated marked accumulation, GSK461364 displayed cytotoxicity against all the three OS cell lines when assessed using either an MTT assay, or a trypan blue exclusion assay cells at G2/M DNA content in all 3 OS cell lines.A significant increase in SA-β-gal activity in the U2OS cells was revealed following GSK461364 treatment.Furthermore, the expression of IL-1α, a cytokine associated with the senescence-associated secretory phenotype (SASP) (34,36), was upregulated in the U2OS cells treated with GSK461364, as measured through qRT-PCR. |
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Regulatory relationship
| R-EF-Target gene:
|
Downregulation |
| Target gene experiment: |
Western blot |
| Target gene description: |
Decreased level of PLK1 and pCDC25C were noted in all three GSK461364-treated OS cell lines. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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