External factors
| External factors: |
T-oligo |
| Category: |
Chemical compounds |
| Phenotype: |
Colorectal cancer |
| Experiment: |
SA-β-gal activity assay//Cell morphological analysis |
| Description: |
44.8% of cells treated with T-oligo were senescence associated β-galactosidase (SA β-gal) positive compared to only 3.9% and 4.5% of cells treated with diluent and C-oligo, respectively. T-oligo-treated cells were also larger in size, which is characteristic of cells undergoing senescence. T-oligo increased expression of senescent markers p53 (2.0-fold), p27 (1.8-fold), and p21 (2.0-fold). |
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Regulatory relationship
| R-EF-Target gene:
|
Downregulation//Downregulation |
| Official symbol(s): |
POT1//TERF2 |
| Target gene experiment: |
Western blot//Immunfluorescence |
| Target gene description: |
We demonstrated that T-oligo treatment downregulated TRF2 in LoVo (2.4-fold, p<0.01) and HT-29 (1.8-fold (p<0.02) cells compared to C-oligo treatment as assessed by immunfluorescence. Additionally, T-oligo treatment downregulates POT1 in both HT-29 (2-fold) and LoVo (3-fold) cells at 48 h and 72 h, respectively as seen by immunoblotting. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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