External factors
| Experiment: |
Western blot//BrdU assay//Colony formation assay//Cell morphological analysis//SA-β-gal activity assay//SAHF |
| Description: |
IRGs induced a stable cell cycle arrest, as determined by a reduction in cyclin A, the phosphorylation status of RB, and 5-bromo-2′-deoxyuridine (BrdU) inco -rporation.The number of colony-forming cells after 2-wk incubation with compound-free medium was strongly reduced if they were pretreated with IRGs.Cells pretreated with the IRGs typically showed an enlarged cellular morphology with increased SA-β-gal activity. p53 and its target p21 were stably upregulated in IRG-treated cells. the levels of HMGA2(a senescence marker) were increased only at the later time point. SAHF formation was also more evident at d9. |
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Regulatory relationship
| Target gene:
|
AURKA//AURKB |
| R-EF-Target gene:
|
Downregulation//Downregulation |
| Official symbol(s): |
AURKA//AURKB |
| Target gene experiment: |
Western blot |
| Target gene description: |
All five IRGs exhibited a substantial inhibitory effect against AURKA and AURKB, with stronger effects on AURKB. |
| R-EF-Pathway:
|
Upregulation |
| Official symbol(s): |
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TP53
|
| Pathway experiment: |
Western blot |
| Pathway description: |
Of interest, we found that the p53-p21 pathway and HMGA2 were up-regulated, particularly at d9 . |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.