External factors
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//Flow cytometry//Colony formation assay |
| Description: |
BAPTA application impeded H2O2-induced increase of the cell size as well as SA-β-Gal activity, indicating some modulation of the senescence phenotype.BAPTA treatment led to marked increase in the number of proliferating cells compared to H2O2-stimulated cells, indicating that Ca2+ chelation overcame the growth arrest induced by H2O2. |
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Regulatory relationship
| R-EF-Target gene:
|
Downregulation |
| Target gene experiment: |
Flow cytometry//Western?blot |
| Target gene description: |
Loading of H2O2-stimulated cells with BAPTA led to a noticeable decrease in the intracellular ROS levels. Indeed, loading with BAPTA dramatically reduced phosphorylation of each DDR participant as compared to H2O2-treated cells over the entire observation period. |
| Regulatory pathway:
|
p53-p21-Rb |
| R-EF-Pathway:
|
Downregulation |
| Official symbol(s): |
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TP53-CDKN1A
|
| Pathway experiment: |
Western blot |
| Pathway description: |
Accordingly, in H2O2-treated hMESCs, calcium chelation significantly attenuated phosphorylation of p53, prevented enhanced p21 protein expression and elevated the Rb phosphorylation levels long after senescence induction . |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.