External factors
| External factors: |
Glucose |
| Category: |
Chemical compounds |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay |
| Description: |
As hypothesized, HG caused notable increases in SA β-gal staining, further confirming our previous report of glucose-induced acceleration of EC senescence . |
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Regulatory relationship
| R-EF-Target gene:
|
Downregulation |
| Target gene experiment: |
Western blot//qRT-PCR//ELISA |
| Target gene description: |
HG exposure for 48?hr markedly reduced the mRNA expressions of all mitochondrial SIRTs. No such changes were seen following incubation with 25? mmol/L L-glucose (osmotic control). We further measured protein levels of these SIRTs using ELISA. In keeping with mRNA levels, protein levels of SIRTs 3, 4, and 5 were also significantly reduced under HG environments. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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