External factors
| External factors: |
Lidamycin |
| Category: |
Chemical compounds |
| Phenotype: |
Colorectal cancer |
| Experimental category: |
HL |
| Cell name: |
Colon cancer cell |
| Experiment: |
SA-β-gal activity assay |
| Description: |
Significant induction of senescent phenotype was observed time dependently after 0.5 nM LDM treatment for 48 120 h, with the ratio of senescent cells reaching over 60% in both cells on day 5. Lower dose (0.25 nM) LDM also caused significant senescence. |
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Regulatory relationship
| R-EF-Target gene:
|
Downregulation |
| Target gene experiment: |
Western blot//RT-PCR |
| Target gene description: |
Similar reductions of EZH2, EED and SUZ12 protein levels were observed by LDM treatment at various doses for 72 h .Further RT-PCR analysis confirmed significant reduction of EZH2 mRNA upon LDM treatment time dependently. |
| R-EF-Pathway:
|
Upregulation |
| Official symbol(s): |
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CDKN1A
|
| Pathway experiment: |
Western blot |
| Pathway description: |
Consistent with our previous work,12 p53 is found to be increased after LDM treatment for 48 120 h in HCT116 cells harboring wild-type p53. Meanwhile, p53 downstream target p21 was increased. Although little changes of p53 expression were seen in SW620 cells carrying mutant p53, p21 level remained to be enhanced. The same tendency was also found dose dependently. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.