External factors
| External factors: |
zofenoprilat |
| Category: |
Chemical compounds |
| Cell name: |
CVEC,FGF-2(-/-) |
| Experiment: |
SA-β-gal activity assay |
| Description: |
Zofenoprilat inhibits endothelial senescence.CVEC were incubated with different concentrations of zofenoprilat (1–10uM) and FGF-2 (20 ng/ml) for 3 days. Cell senescence was evaluated by SA-β-gal staining. Representative pictures of SA-β-gal-positive cells (blue) are reported. After 3 days in culture, the number of SA-β-gal-positive cells in a total of 400 cells was counted (n=3). |
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Regulatory relationship
| R-EF-Target gene:
|
Activation//Upregulation |
| Official symbol(s): |
FGF2//TERT |
| Target gene experiment: |
qRT-PCR//Western blot |
| Target gene description: |
Zofenoprilat increased TERT mRNA (6-fold), approaching the level reached by FGF-2 (20 ng/ml).whereas that produced by FGF-2 was rapid and declined with time. |
| Regulatory pathway:
|
Akt//eNOS |
| R-EF-Pathway:
|
Activation//Activation |
| Official symbol(s): |
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AKT1//NOS3
|
| Pathway experiment: |
qRT-PCR//Western blot |
| Pathway description: |
Zofenoprilat (10um) enhanced the phosphorylation of Akt . This activation was slow but persistent (up to 6 h).Assessment of eNOS activation downstream of Akt, measured by phosphorylation of eNOS in Ser1177, demonstrated that zofenoprilat increased eNOS activation . |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.