External factors
| External factors: |
Hyperphosphatemia |
| Experiment: |
Western blot//SA-β-gal activity assay |
| Description: |
Results showed a significant increased expression in all proteins （p53, p21 and p16）after 24 h of treatment；Senescence was also analyzed, in the same experimental conditions,by the SA-β-gal activity, using a fluorescent probe, by confocal microscopy. A strong fluorescent signal was found after 24 h of treatment which was progressively increasing until 72 h. |
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
383
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Regulatory relationship
| Target gene:
|
INTEGRIN LINKED KINASE (ILK) |
| R-EF-Target gene:
|
Upregulation |
| Official symbol(s): |
INTEGRIN LINKED KINASE (ILK) |
| Target gene experiment: |
Western blot |
| Target gene description: |
ILK protein expression was analyzed in HASMC treated with 10 mM BGP during 24, 48 or 72 h and results showed a significant increase after 24 h of treatment, which remained until 72 h. BGP also increased ILK activity, measured as the GSK-3β phosphorylation. |
| Regulatory pathway:
|
IGF1-Akt-FoxO |
| Official symbol(s): |
Warning: Undefined variable $name_all in /data/html/Aging-ReG/public/browse_detail_2.php on line 525
IGF1-AKT
|
| Pathway experiment: |
Western blot |
| Pathway description: |
We analyzed whether BGP treatment activated the IGF-1 pathway,by evaluating the IGF-1R phosphorylation,and found a sustained increase from 24 to 72h .significant increase in AKT phosphorylation at Ser473 was observed after BGP treatment.Results showed that BGP additionincreased FoxO1 phosphorylation andtotal FoxO1 expression from 24-72h after treatment . |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.