External factors
| External factors: |
20(s)-Rg3 |
| Category: |
Chemical compounds |
| Phenotype: |
Gallbladder cancer |
| Experiment: |
SA-β-gal activity assay//Western blot//Cell morphological analysis |
| Description: |
Morphological changes of NOZ and GBC-SD cells could be seen. Both cell lines became enlarged and flattened, had more cytoplasmic vacuoles in a concentration-dependent manner, representing a senescence-like state.The higher the concentration of 20(S)-Rg3, the more SA-β-gal positive cells in both cell lines was observed. Moreover, the protein amounts of p53 and p21Cip1 increased in senescent cells but not the p16 and pRB protein . |
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Regulatory relationship
| R-EF-Target gene:
|
Downregulation |
| Target gene experiment: |
Western blot |
| Target gene description: |
Western blot further showed that MDM2 protein levels were decreased by 20(S)-Rg3 treatment, in a dose- and time-dependent manner. |
| Official symbol(s): |
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TP53
|
| Pathway experiment: |
Western blot |
| Pathway description: |
We next investigated the involvement of the transcription-independent function of p53 using a protein synthesis inhibitor, (CHX), and found the suppressive effect of CHX on p53 protein accumulation was neutralized by 20(S)-Rg3 . |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.