External factors
| External factors: |
PD-0332991 |
| Category: |
Chemical compounds |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay |
| Description: |
To establish senescence, presenescent MEFs (passage 4/p4) were treated with PD-0332991 (4 mmol/L for 8 days),Herein, these treated fibroblast populations are referred to as CDK4/6i.Next, we measured SA-β-gal positivity in these cells, revealing that 74% CDK4/6i treated cells were SA-β-gal positive. |
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Regulatory relationship
| Target gene:
|
MDM2//P53//P21/WAF1 |
| R-EF-Target gene:
|
Downregulation//Downregulation//Upregulation |
| Official symbol(s): |
MDM2//P53//P21/WAF1 |
| Target gene experiment: |
Western blot |
| Target gene description: |
We found that prolonged PD-0332991 treatment promoted a reduction in Mdm2 and p53 protein levels. However, unlike what is observed in liposarcomas, p21Cip1/Waf1 protein levels were elevated in CDK4/6i MEFs in comparison to p4 cells. |
| Regulatory pathway:
|
NF-κB |
| Official symbol(s): |
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NFKB1
|
| Pathway experiment: |
Western blot |
| Pathway description: |
In contrast, activating phosphorylation of the NF-kB subunit, p65, was elevated in CDK4/6i, UV, and MMC fibroblasts when compared with control. |
Aging network
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Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.