External factors
| External factors: |
Curcumin |
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//Flow cytometry |
| Description: |
Pretreatment with curcumin at 10 and 25 μM? could? significantly? attenuate? the? H2O2-induced HUVECs? senescence? (54.40±3.40% vs. 82.90±2.02%; 51.25±2.74% vs. 82.90±2.02%, p<0.01).Pretreatment of 10 and 25 μM?curcumin?remarkably?decreased?the?ratio of cells at G0/G1 stage (60.02±5.22% vs. 78.12±2.91% and 56.57±4.15% vs. 78.12±2.91%, p<0.05). Accordingly, the ratio of cells in S and G2/M?phases?were?increased?in?curcumin pretreated group compared to the H2O2 group (p<0.05). |
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Regulatory relationship
| R-EF-Target gene:
|
Activation//Downregulation |
| Official symbol(s): |
SIRT1//P21 |
| Target gene experiment: |
Western blot |
| Target gene description: |
100?μM H2O2 remarkably?decreased?the?expression?of?SIRT1?and?pretreatment with curcumin could increase the expression of SIRT1 in H2O2-treated? HUVECs?. Interestingly, treatment of curcumin alone greatly improved the enzymatic activity of SIRT1 and pretreatment of curcumin could also reverse the change of enzymatic activity of SIRT1 in H2O2-treated? HUVECs?.Meanwhile, pretreatment of 25 μM? curcumin? significantly?decreased the expression of p21 in H2O2-treated?HUVECs?. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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