External factors
| External factors: |
High Glucose |
| Phenotype: |
Diabetes kidney disease |
| Experiment: |
Flow cytometry |
| Description: |
The results showed that high glucose led to the hypertrophy of HGMCs and decreased cell number. HG group of HGMCs had cell cycle arrest in G1 phase (P<0.05). |
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
383
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Regulatory relationship
| R-EF-Target gene:
|
Downregulation |
| Official symbol(s): |
MIR-126 |
| Target gene experiment: |
Flow cytometry//qRT-PCR |
| Target gene description: |
Flow cytometry was used to detect the cell cycle, and the results displayed that miR-126 transfection resulted in a decrease in the ratio of cell arrest in the G1 phase (P<0.01)The miR-126 expression in HG group (5.5±0.8) was significantly lower than that in the NG group. |
| Regulatory pathway:
|
Telomere-p53-p21-Rb |
| Official symbol(s): |
Warning: Undefined variable $name_all in /data/html/Aging-ReG/public/browse_detail_2.php on line 525
RTEL1-TP53-CDKN1A-RB1
|
| Pathway experiment: |
Southern blot//Western blot |
| Pathway description: |
The osmotic pressure had no significant effect on the telomere TRF length of cells. The telomere TRF length of HGMCs in NG group and HG group was (8.97±0.35) and (6.77±0.65) kb respectively. As compared with NG group, the telomere TRF length in HG group was significantly shortened (P<0.01).The Western blotting results showed that high glucose-induced expression of p53, p21, and Rb in HGMCs was significantly higher than that in NG group . |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
Related pathway view