External factors
| External factors: |
DNA damage |
| Experimental category: |
HL |
| Experiment: |
BrdU assay//SA-β-gal activity assay |
| Description: |
Cells were challenged in the absence or presence of 10 μM etoposide for 24 h to induce DNA damage,Brdu assay:As expected, FaDu cells displayed several markers of senescence, such as cell flattening, increase in cellular size, significantly decreased 5-bromodeoxyuridine (BrdU) incorporation.SA-β-gal activity assay:and a positive senescence-associated-β-galactosidase (SA-β-Gal) staining in response to etoposide. 66% of FaDu cells stained positive for SA-β-Gal seven days post-treatment. |
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Regulatory relationship
| R-EF-Target gene:
|
Downregulation |
| Official symbol(s): |
MYBBP1A |
| Target gene experiment: |
BrdU assay//SA-β-gal activity assay//Knockdown |
| Target gene description: |
Knockdown//Brdu assay: it resulted in a slight but significantly decreased amount of BrdU positive cells. Knockdown//SA-β-gal activity assayand induced the relative number of senescent tumor cells at day 5 post-treatment (2–3 fold) as determined by SA-βgal-positive staining. |
| Regulatory pathway:
|
PI3K-Akt |
| Official symbol(s): |
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PIK3CB-AKT
|
| Pathway experiment: |
Western blot |
| Pathway description: |
A strong increase in phosphorylation of AKT (pAKT(Ser473)) was detected in response to etoposide by Western blot analysis, which was consistent with previous observation. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.