External factors
| External factors: |
Lactate |
| Category: |
Chemical compounds |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay//Cell cycle analysis//Immunofluorescence |
| Description: |
Remarkably, lactate decreased SA-β-gal activity in a dose-dependent manner.In contrast, the accumulation of γH2AX positive cells was significantly decreased upon lactate treatment.However, the majority of lactate-treated B2B/K-Ras showed a cell cycle progression though G2/M, as evident by accumulation of 4 N DNA content. |
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Regulatory relationship
| R-EF-Target gene:
|
Upregulation//Downregulation |
| Official symbol(s): |
SNAIL//P16 |
| Target gene experiment: |
qRT-PCR//Dual-Luciferase reporter assay |
| Target gene description: |
Notably, lactate markedly upregulated Snail mRNA in a dose-dependent manner in both A549 and H1299 cells paralleled with obtained with Snail protein levels . Importantly, treatment with lactate attenuated p16INK4a expression activated by K-Ras oncogene. This finding is not oncogenic K-Ras specific, similar results were obtained with transfection of Activate B-Raf (V600E) and dominant negative PTEN(C124S) into B2B cells.qRT-PCR showed that lactate dose-dependently decreased p16INK4a mRNA levels in B2B cells Notably, the promoter activity of p16INK4a was significantly reduced by lactate treatment, indicating that lactate transcriptional regulate p16INK4a expression. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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