External factors
| External factors: |
Angelica sinensis Polysaccharides |
| Category: |
Chemical compounds |
| Phenotype: |
Acute Leukemia |
| Experiment: |
SA-β-gal activity assay//Southern blot |
| Description: |
After 40 μg/ml ASP treatment for 48h, the staining positive cells are much more than CD34+CD38? cells absence of ASP performed by SA-β-Gal staining assay.The telomere lengths of AML CD34+CD38? cells chromosome ends have been sharply shortened after ASP treatment presented by Southern Blotting. |
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Regulatory relationship
| Target gene:
|
P16//P21//CYCLIN E//CDK4 |
| R-EF-Target gene:
|
Upregulation//Upregulation//Downregulation//Downregulation |
| Official symbol(s): |
P16//P21//CYCLIN E//CDK4 |
| Target gene experiment: |
Western blot |
| Target gene description: |
As a result, increased level expression of P16INK4a and P21 but decreased level of CDK4 and CyclinE were observed after 40 μg/ml ASP treatment for 48 h . |
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| Regulatory pathway:
|
p53-p21//p16-pRb |
| R-EF-Pathway:
|
Upregulation//Upregulation |
| Official symbol(s): |
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TP53-CDKN1A//
|
| Pathway experiment: |
qRT-PCR |
| Pathway description: |
The expressions of these genes have remarkably been enhanced due to ASP treatment, indicating that p53/p21 and p16INK4a/pRb pathways play an important role in the process of ASP-induced AML CD34+CD38? cells senescence. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
Pathway view
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