External factors
| External factors: |
Palbociclib |
| Category: |
Chemical compounds |
| Phenotype: |
Malignant pleural mesothelioma |
| Experiment: |
Cell cycle analysis//Cell morphological analysis//SA-β-gal activity assay//Western blot |
| Description: |
Palbociclib induced a significant blockade of the progression from G1 to S phase of the cell cycle in all MPM sensitive cells; Interestingly, treatment with palbociclib induced an accumulation of senescent-like cells, characterized by the production of the Senescent-Associated β-galactosidase enzyme (SA-β-Gal) and by flat and enlarged cell morphology;In addition, the level of cyclin D1 was increased, confirming cyclin D1 induction as a marker associated to cellular senescence. |
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
383
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Regulatory relationship
| R-EF-Target gene:
|
Upregulation |
| Target gene experiment: |
Western blot |
| Target gene description: |
We observed a great increase in p21 expression,that might be ascribed to the release of p21 gene negative regulation exerted by myc, or to AKT/mTOR signaling activation, which has been shown to initiate p21-dependent senescence in particular conditions. |
| Regulatory pathway:
|
Akt-mTOR |
| Official symbol(s): |
Warning: Undefined variable $name_all in /data/html/Aging-ReG/public/browse_detail_2.php on line 525
AKT1-MTOR
|
| Pathway experiment: |
Western blot |
| Pathway description: |
The additive/synergistic inhibitory effects of the drug combination on cell proliferation were associated with a significant downregulation of p-CDK6, p-Rb, Rb levels, as well as with the inhibition of the AKT/mTOR pathway in both MSTO and ZS-LP cells. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.