External factors
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//Flow cytometry//Cell morphological analysis |
| Description: |
Cell cycle analysis revealed that the treatment of P388 cells with F14512 or etoposide resulted in S-phase accumulation at 4–8 h of treatment whereas the proportion of cells in G1 phase decreased.P388 cells positive for SA-β-gal demonstrated enlarged morphology.Using this flow cytometric method for quantitative analysis of senescent cells, we observed that F14512 was more successful in inducing premature senescence of P388 cells than etoposide. |
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Regulatory relationship
| R-EF-Target gene:
|
Upregulation |
| Official symbol(s): |
SURVIVIN |
| Target gene experiment: |
Western blot//Immunofluorescence |
| Target gene description: |
Immunofluorescence experiments revealed a pronounced increase of survivin level in the nucleus and to a lesser extent in the cytoplasm of F14512 or etoposide treated cells .Exposure of cancer cells to F14512 or etoposide resulted in a striking increase in survivin expression by western blotting. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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