External factors
| Category: |
Chemical compounds |
| Cell name: |
LN-229-MGMT,U87,LN-229 |
| Experiment: |
Flow cytometry//PI staining//SA-β-gal activity assay |
| Description: |
The data revealed that repeated low-dose TMZ exposure is highly efficient in inducing senescence in p53 proficient U87 (up to 60%) and LN229 (up to 80%) cells .Compared to the high level of senescence, apoptosis and necrosis were induced at low levels (up to 20%) upon repeated treatment of U87 and up to 10% necrosis and 25% apoptosis upon single exposure. Also in LN229 cells repeated treatment induced up to 20% apoptosis and necrosis and single exposure induced up to 15% apoptosis and necrosis . |
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Regulatory relationship
| Official symbol(s): |
P21//NFKB1 |
| Target gene experiment: |
SA-β-gal activity assay//Flow cytometry |
| Target gene description: |
In line with this, knockdown of p21 completely abrogated the induction of senescence , The results showed a dramatic reduction in temozolomide-induced senescence following NF-κB inhibition . |
| Regulatory pathway:
|
MRN-ATR |
| Official symbol(s): |
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ATR
|
| Pathway experiment: |
SA-β-gal activity assay |
| Pathway description: |
To analyze whether this is also true for the activation of senescence, the DDR factors ATM, ATR, MRN, and DNA-PKcs were pharmacologically inhibited and the frequency of senescence was measured in LN229 cells (proficient for p53 and deficient for MGMT) by scoring senescence-associated β-galactosidase (β-Gal)–positive cells. To identify the senescence-inducing DNA lesions, activation of senescence was also measured in LN229 cells that express MGMT following transfection with MGMT cDNA (LN229-MGMT). The data show that senescence was induced only in MGMT-deficient LN229 glioma cells, but not in the MGMT-proficient LN229-MGMT isogenic cell line. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.