External factors
| External factors: |
Cigarette smoke |
| Phenotype: |
Chronic obstructive pulmonary disease |
| Experiment: |
SA-β-gal activity assay//Western blot |
| Description: |
As expected, we found that CSE (0.5%) treatment for both 15 days and 30 days induced cellular senescence, which is characterized by increased senescence-associated β-galactosidase (SA-β-gal) activity, p16 (CDKN2A or INK4A) and p21 (CDKN1A) protein abundance. |
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Regulatory relationship
| R-EF-Target gene:
|
Downregulation//-- |
| Official symbol(s): |
TPP1//SIRT1 |
| Target gene experiment: |
Western blot//Immunofluorescence |
| Target gene description: |
Nuclear abundance of TPP1 and TIN2 was reduced with CSE .We performed coimmunoprecipitation of TPP1 with or without CSE (0.5% for 15 days) in HFL1 cells, and probed with acetyl lysine and the Sirt1 antibody. Similarly, Sirt1 pull-down was performed and the lysate was probed for the TPP1 antibody. This was further confirmed by co-immunofluorescence for Sirt1 and TPP1 in HFL1 cells. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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