External factors
| Category: |
Chemical compounds |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay//qRT-PCR//Western blot |
| Description: |
The mRNA and protein levels of each MCM declined as a function of time in culture.However, HU treatment triggered more severe senescence-associated phenotypes in M2 than WT primary MEFs, both in terms of markedly higher p16Ink4a/p19ARF induction and a ~2 fold increase in cells positive for SA-β-gal. |
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Regulatory relationship
| R-EF-Target gene:
|
Upregulation |
| Official symbol(s): |
TRP53 |
| Target gene experiment: |
qRT-PCR |
| Target gene description: |
We confirmed the sequencing results using miRNA qRT-PCR. Interestingly, Trp53 deletion abolished the HU induced miRNA upregulation in the WT primary MEFs, confirming these candidate miRNAs are Trp53-dependent. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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