External factors
| External factors: |
G-quadruplex ligand 12459 |
| Category: |
Chemical compounds |
| Experiment: |
Western blot//Immunostaining |
| Description: |
Prolongation of 12459 treatment to 8 days provokes a cell growth arrest where P21 expression became readily detectable, suggesting the onset of cell senescence. Results showed that DNA damage foci induced by 12459 co-localized with TRF1 and corresponded to TIFs (Telomere-dysfunction Induced Foci). |
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Regulatory relationship
| R-EF-Target gene:
|
Upregulation//Activation |
| Official symbol(s): |
P53//CHK1 |
| Target gene experiment: |
Western blot |
| Target gene description: |
Results indicate that 12459 treatment of A549 cells(0.5–2mM) induces a transient overexpression of p53 after 4 days.In contrast, a significant activation of Chk1(Ser317) was found at the onset of senescence after 8 days that coincides with the expression of p21. |
| Regulatory pathway:
|
PPM1D-WIP |
| Official symbol(s): |
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PPM1D-WIPF1
|
| Pathway experiment: |
Western blot |
| Pathway description: |
The expression of PPM1D/WIP1 was thus analyzed by western blot during the time-course induction of p53 by 12459 . Results show that PPM1D/WIP1 expression is activated by 12459 treatment and starts after 2 h. An increased level of PPM1D/WIP1 is maintained up to 48h. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.