External factors
| External factors: |
BTM-0512 |
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay |
| Description: |
A trend for increased senescence, although non-significant, could be observed at 24 h after treating the endothelial cells with D-glucose (30 mmol ? L), which reached the significant level at 48 h after the treatment. The high glucose-induced senescence was significantly reduced in the presence of BTM-0512 at a concentration of more than 3 lmol ? L. L-glucose (30 mmol ? L) and the vehicle (0.1% alcohol) had no effect on cell senescence. |
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Regulatory relationship
| R-EF-Target gene:
|
Activation |
| Official symbol(s): |
SIRT1 |
| Target gene experiment: |
RT-PCR |
| Target gene description: |
Moreover, BTM-0512 also reversed the high glucose-induced downregulation of SIRT1 mRNA expression. |
| Regulatory pathway:
|
DDAH-ADMA |
| Official symbol(s): |
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DDAH1
|
| Pathway experiment: |
qPCR//Western blot |
| Pathway description: |
Administration of BTM-0512 alone dramatically upregulated DDAH2 mRNA expression and DDAH activity .Transfection of HUVEC with PGCsi-DDAH2-shRNA successfully knocked down the DDAH2 mRNA expression, reduced the DDAH activity and increased the level of ADMA in the cultured medium . |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.