External factors
| External factors: |
Amyloid beta oligomer |
| Experiment: |
Cell morphological analysis//SA-β-gal activity assay |
| Description: |
S1A and S1B, HBMECs exposed to Aβ1–42 oligomers showed increased senescence-associated β-galactosidase staining (> 60% compared to ~23% in controls), confirming a senescent phenotype.Using optical microscopy, we observed that the morphology of β-galactosidase positive Aβ1–42 oligomer treated HBMECs displayed a mixed population of normal appearing cells as well as enlarged cells with a flatter and spread-out nucleus, the latter being a characteristic feature of senescent cells. |
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Regulatory relationship
| Target gene:
|
VEGFR-1//RAC1 |
| R-EF-Target gene:
|
Upregulation//Downregulation |
| Official symbol(s): |
FLT1//RAC1 |
| Target gene experiment: |
Western blot |
| Target gene description: |
Furthermore, we showed that VEGFR-1 was highly upregulated in HBMECs following treatment with Aβ1 42 oligomers and knockdown of VEGFR-1 significantly reduced Aβ1 42 oligomerinduced senescence in the HBMECs, suggesting a key role of VEGRF-1 expression and signaling in this paradigm.Quantification of the western blot showed a significant reduction in Rac 1 protein levels in the senescent HBMECs (~40 50% reduction compared to untreated controls. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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