External factors
| External factors: |
Inauhzin |
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//Knockdown |
| Description: |
INZ induced senescence in H460 or HCT116p53+/+, but not in HCT116p53?/?, cells, though to a much less degree than did Nutlin-3. |
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Regulatory relationship
| R-EF-Target gene:
|
Downregulation |
| Official symbol(s): |
SIRT1 |
| Target gene experiment: |
Western blot |
| Target gene description: |
INZ inhibited SIRT1 deacetylase activity in a dose‐dependent fashion and effectively inhibited this activity at 3?μM. This inhibition was specific to INZ and its chemical analogue INZ1 (methyl substituted R1), which activated p53 and decreased SIRT1 activity in a dose‐dependent fashion. |
| R-EF-Pathway:
|
Upregulation |
| Official symbol(s): |
Warning: Undefined variable $name_all in /data/html/Aging-ReG/public/browse_detail_2.php on line 525
TP53
|
| Pathway experiment: |
Western blot |
| Pathway description: |
We found that INZ induced p53 level in a time-dependent manner as early as 6 h post-treatment in both p53-containing H460 and HCT116 cells. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.