External factors
| External factors: |
Glucocorticoid |
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay |
| Description: |
By 48 and 72 h post-treatment, a significant increase in the percentage of SA-β-gal-positive cells was observed. |
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Regulatory relationship
| R-EF-Target gene:
|
Downregulation |
| Official symbol(s): |
SIRT1 |
| Target gene experiment: |
qRT-PCR//Western blot |
| Target gene description: |
We found a small but significant reduction in RNA levels of sirt1, but a marked reduction in protein levels in glucocorticoid-treated cells compared with carrier-treated controls. |
| Official symbol(s): |
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TP53
|
| Pathway experiment: |
Western blot |
| Pathway description: |
In contrast, levels of acetylated p53 (but not phosphorylated p53) were higher in glucocorticoid-treated tenocytes compared with controls. While there were no significant differences in p53 RNA levels, RNA levels of p21cip/waf1, a pro-senescence modulator of p53 activity, were markedly elevated post-glucocorticoid treatment . |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.