External factors
| External factors: |
Sirtinol |
| Category: |
Chemical compounds |
| Experiment: |
BrdU assay//SA-β-gal activity assay//Western blot//Colony formation assay |
| Description: |
Treatment with Sirtinol inhibited cell growth in both MCF-7 and H1299 cells；This was supported by reduced incorporation of BrdU in Sirtinol-treated MCF-7 and H1299 cells at 10 days after the addition of Sirtinol, as compared with untreated cells；Sirtinol treatment increased SA-β-gal-positive cells in a dose-dependent manner 10 days after the addition of Sirtinol in both MCF-7 and H1299 cells；Sirtinol treatment also resulted in increased expression of PAI-1 in both MCF-7 and H1299 cells.Colony formation assay also revealed that both Sirtinol and Splitomicin elicited antiproliferative effects in MCF-7 and H1299 cells in a dose-dependent manner. |
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Regulatory relationship
| R-EF-Target gene:
|
Downregulation |
| Official symbol(s): |
SIRT1 |
| Target gene experiment: |
Cell morphological analysis//SA-β-gal activity assay |
| Target gene description: |
Sirt1 inhibition by Sirtinol, Splitomicin or siRNA also induced senescence-like phenotype in human diploid fibroblasts, WI-38 and IMR-90 cells, reflected by induction of SA-β-gal staining, and enlarged and flattened cell morphology. |
| Regulatory pathway:
|
Ras-MAPK |
| Official symbol(s): |
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MAPK
|
| Pathway experiment: |
Western blot |
| Pathway description: |
By contrast, in Sirtinol-treated senescent MCF-7 and H1299 cells at 10 days after the addition of Sirtinol (100mM), basal (unstimu-lated) phosphorylation of ERK, JNK/SAPK and p38 MAPK was reduced compared with untreated cells. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.