External factors
| External factors: |
HIV protease inhibitors |
| Experiment: |
SA-β-gal activity assay//Western blot//Cell morphological analysis |
| Description: |
We tested senescence markers in PI-treated VSMCs and observed that SA-β-galactosidase activity was increased by 3- to 4-fold in LPV/r- and ATV/r-treated cells versus DMSO .The protein expression of the senescence markers, p16INK4, p21WAF, p53 and phospho-p53 was also altered .LPV/r-treated VSMCs harbored the typical morphology of senescent cells, with an increased number of prelamin A-stained nuclei showing dysmorphies, and of foci containing phosphorylated histone γ-H2AX indicating DNA damages. |
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Regulatory relationship
| R-EF-Target gene:
|
Downregulation |
| Official symbol(s): |
ZMPSTE24 |
| Target gene experiment: |
Western blot |
| Target gene description: |
We first evaluated the expression of miR-141-3p, which was shown by Yu et al. to regulate ZMPSTE24 protein expression during cellular senescence [40]. The level of miR-141-3p was increased by 1.5-fold in VSMCs expressing D47Y and R482W LMNA mutant and by 2-fold in LPV/r-treated cells, as compared to control cells. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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