External factors
| External factors: |
Regulatory T cells |
| Cell name: |
CD4+CD25?T,CD8+T,B,NK |
| Experiment: |
TRAP assay//Flow cytometry//SA-β-gal activity assay |
| Description: |
Using the TRAP assay, we found that both nTregs and iTregs could induce the inhibition of telomerase activity in all the target cells.Upon co-cultivation with Tregs, we observed a significant decrease in the number of target cells in the S and G2/M phases and a reliable increase in the number of cells in the G0/G1 phases 30 days after transfection. β–Gal expression levels remained stably high over the days 60. β–Gal enzymatic activity also increased 45 days after transfection and remained high on day 60. |
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
383
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Regulatory relationship
| R-EF-Target gene:
|
Upregulation |
| Official symbol(s): |
α+β+HTERT |
| Target gene experiment: |
Western blot//RT-PCR |
| Target gene description: |
Western blotting results showed a significant decrease in full-length α+ β+ hTERT and an increase in α+β– hTERT and EndoG in the target cells co-cultured with nTregs or iTregs Real-time RT-PCR revealed that cultivation of the target cells in the absence of iTregs resulted in a gradual increase in α+ β+ hTERT expression, decrease in α+ β– hTERT expression and decrease in EndoG expression to the levels of the control target cells over 48 h. |
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
471
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
472
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
Warning: Trying to access array offset on null in /data/html/Aging-ReG/public/browse_detail_2.php on line 751
Warning: Trying to access array offset on null in /data/html/Aging-ReG/public/browse_detail_2.php on line 754
Warning: Undefined variable $pathway_id in /data/html/Aging-ReG/public/browse_detail_2.php on line 793