External factors
| External factors: |
HIV Tat |
| Description: |
Expression levels of GFAP(astrocyte activation as an indicator of aging) were significantly increased with aging in the both the WT and Tg rats. |
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
383
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
383
Regulatory relationship
| Target gene:
|
SIRT1//MIR-34A//MIR-138 |
| R-EF-Target gene:
|
Downregulation//Upregulation//Upregulation |
| Official symbol(s): |
SIRT1//MIR-34A//MIR-138 |
| Target gene experiment: |
Western blot//qRT-PCR |
| Target gene description: |
A significant decrease in SIRT1 protein content was detected in both A172 and human primary astrocytes following stimulation with HIV Tat;And in keeping with the in vivo data, there was increased expression of miRs-34a & -138 in the both cell line and primary astrocytes treated with HIV Tat compared with untreated control cells. |
| Regulatory pathway:
|
NF-κB |
| R-EF-Pathway:
|
Upregulation |
| Official symbol(s): |
Warning: Undefined variable $name_all in /data/html/Aging-ReG/public/browse_detail_2.php on line 525
NFKB1
|
| Pathway experiment: |
Western blot |
| Pathway description: |
Pretreatment of A172 cells with Ikk-2 inhibitor SC514 (5 μM) significantly decreased HIV Tat-mediated induction of GFAP.This was further confirmed by the fact that transfection of A172 cells with mutant IκB resulted in amelioration of HIV Tat-mediated induction of GFAP . |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.