External factors
| External factors: |
Doxorubicin |
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay |
| Description: |
Thus in order to induce senescence we treated BJ cells with 50 and 100 ng/ml of doxorubicin for 5 days as suggested in literature [38]. Induction of senescence was evident with increased SA-β-gal activity, increased levels of p53 and p21CIP1 and γ-H2A.X foci formation. |
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Regulatory relationship
| Target gene:
|
SIRT1//SIRT3 |
| R-EF-Target gene:
|
Downregulation//Downregulation |
| Official symbol(s): |
SIRT1//SIRT3 |
| Target gene experiment: |
Western blot |
| Target gene description: |
Remarkably WB analysis showed that expressions of SIRT1/2 were also slightly reduced during doxorubicin induced senescence. |
| Regulatory pathway:
|
p53-p21 |
| Official symbol(s): |
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TP53-CDKN1A
|
| Pathway experiment: |
SA-β-gal activity assay//Western blot |
| Pathway description: |
Additionally, when we tested p16 INK4A levels we found rather minor increase in p16INK4A levels suggesting doxorubicin induced senescence is mediated mainly by activation of p53-p21 pathway . |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.