External factors
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay |
| Description: |
The results showed that the percentage of cells stained by X?gal following 9 J/cm2 UVA irradiation was markedly increased compared with that in the control group (53 and 8%, respectively; P<0.05), while 50 ng/ml PQQ attenuated the ratio of positive staining compared with that of the UVA-only cells (29 vs. 53%, respectively; P<0.01) . |
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Regulatory relationship
| Target gene:
|
SIRT1//SIRT6 |
| R-EF-Target gene:
|
Upregulation//Upregulation |
| Official symbol(s): |
SIRT1//SIRT6 |
| Target gene experiment: |
Western blot//qRT-PCR |
| Target gene description: |
At 72 h following the addition of 50 ng/ml PQQ to the culture media of UVA-irradiated HDFs, mRNA and protein expression levels of SIRT1 and SIRT6 were found to be increased . |
| Regulatory pathway:
|
Nrf2-HO-1 |
| Official symbol(s): |
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NFE2L2-HMOX1
|
| Pathway experiment: |
Western blot//qRT-PCR |
| Pathway description: |
As SIRT1/Nrf2/HO?1 is a classical anti?apoptotic pathway, the present study investigated whether this pathway was involved in UVA-induced cell apoptosis. Altered expression levels of Nrf2 and HO?1 mRNA expression levels were not found to be significant in HDFs pre-treated with 50 ng/ml PQQ without UVA irradiation. However, following UVA irradiation, PQQ was shown to alter the mRNA and protein expression of Nrf2 and HO?1. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.