External factors
| External factors: |
Calorie restriction |
| Experiment: |
SA-β-gal activity assay//ELISA |
| Description: |
SA-β-gal assay is an effective method to measure cell senescence21and significantly fewer SA-β-gal positive cells were observed in CR conditions.Compared with AL group, reduced production of renal TNF-a and IL-1β were observed in mice subjected to CR ,indicated that CR can reduce renal inflammatory activity effectively. |
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Regulatory relationship
| R-EF-Target gene:
|
Upregulation |
| Official symbol(s): |
SIRT6 |
| Target gene experiment: |
Immunostaining//Western blot//SA-β-gal activity assay |
| Target gene description: |
Immunohistochemistry and Western blot analysis of SIRT6 indicated enhanced SIRT6 expression in CR condition. Our results strongly supports the evidence that CR can effectively prevent age-dependent renal failure and promotes in vivo SIRT6 expression.Using SA-β-gal staining, reduced number of SA-β-gal positive cells were observed in WI38 with SIRT6-WT overexpression, but not in cells with SIRT6-HY overexpression. |
| Regulatory pathway:
|
NF-κB |
| R-EF-Pathway:
|
Downregulation |
| Official symbol(s): |
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NFKB1
|
| Pathway experiment: |
DAPI staining |
| Pathway description: |
SIRT6 knockdown induced the translocation of NF-kB p65 into the nucleus along with enlarged cell bodies, indicated an activation of the NF-kB signaling and accelerated cell senescence . |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.