External factors
| External factors: |
Cigarette smoke extract |
| Phenotype: |
Chronic obstructive pulmonary disease |
| Cell name: |
Endothelial cell |
| Experiment: |
Flow cytometry//Colony formation assay//SA-β-gal activity assay//Western blot |
| Description: |
CSE reduced, in a dose dependent manner, short-term cell proliferation;CSE increased beta galactosidase staining;CSE increased p21 expression. |
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Regulatory relationship
| R-EF-Target gene:
|
Upregulation |
| Official symbol(s): |
SURVIVIN |
| Target gene experiment: |
Flow cytometry |
| Target gene description: |
CSE increased, in a dose dependent manner, survivin expression (increase versus baseline of CSE 10% = 13 ± 10; p b 0.01; increase versus baseline of CSE 20% = 26 ± 16; p=0.002). |
| Regulatory pathway:
|
SIRT1-FOXO3 |
| R-EF-Pathway:
|
Downregulation |
| Official symbol(s): |
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SIRT1-FOXO3
|
| Pathway experiment: |
Western blot |
| Pathway description: |
CSE in a dose dependent manner reduced nuclear expression of SIRT1; CSE reduced nuclear expression of FoxO3, in a dose dependent manner. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.