External factors
| External factors: |
Carbocysteine |
| Category: |
Chemical compounds |
| Phenotype: |
Chronic obstructive pulmonary disease |
| Cell name: |
Endothelial cell |
| Experiment: |
Flow cytometry//Colony formation assay//SA-β-gal activity assay//Western blot |
| Description: |
Incubation with CARB significantly counteracted this phenomenon in cells exposed to CSE; CSE increased beta galactosidase staining and incubation with CARB completely reverted this phenomenon;CSE increased p21 expression and CARB counteracted this phenomenon. |
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Regulatory relationship
| R-EF-Target gene:
|
Downregulation |
| Official symbol(s): |
SURVIVIN |
| Target gene experiment: |
Flow cytometry |
| Target gene description: |
A dose dependent manner, survivin expression and the costimulation with CARB significantly counteracted this phenomenon (reduction versus CSE 10% =17 ± 13; p b 0.02 reduction versus CSE 20% = 16 ± 11; p b 0.007). |
| Regulatory pathway:
|
SIRT1-FOXO3 |
| R-EF-Pathway:
|
Upregulation |
| Official symbol(s): |
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SIRT1-FOXO3
|
| Pathway experiment: |
Western blot |
| Pathway description: |
CSE in a dose dependent manner reduced nuclear expression of SIRT1; CSE reduced nuclear expression of FoxO3, in a dose dependent manner. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.