External factors
| Category: |
Chemical compounds |
| Cell name: |
U87,U251,SHG44,C6 |
| Experiment: |
Immunostaining//SA-β-gal activity assay//Telomere length assay//Flow cytometry//Western blot |
| Description: |
Immunofluorescent labeling showed that ATR, 53BP1, γ-H2 AX and Mer11 accumulated in the nucleus of cells exposed to 4 μM As2 O3 for 48h .In addition,obvious dose-related increases in p-ATM, ATR, γ-H2 AX,53BP1, Mer11, and p21 were detected by immunoblotting. As2O3 significantly reduced the telomeric G-overhang length after 48 h of treatment (P < 0.01), though the total telomere length did not change. Most of the cells in the control group are in G0-G1 phase. By contrast, As2O3 induced G2-M phase arrest, which is in agreement with the reported effect of As2O3 on Burkitt’s lymphoma cells .Using aging staining we also observed that As2O3 treatment for 2 weeks increased the incidence of cellular senescence marked by cell swelling and blue staining. Dose-related effects were seen with all of four cell types tested . |
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Regulatory relationship
| R-EF-Target gene:
|
Upregulation//Upregulation |
| Official symbol(s): |
P53//P21 |
| Target gene experiment: |
Western blot |
| Target gene description: |
In addition, obvious dose-related increases in p-A TM, A TR, γ-H2AX, 53BP1, Mer11, and p21 were detected by immunoblotting . Moreover, immunoblotting revealed dose- and time-dependent up-regulation of the pro-apoptotic proteins p53, p-p53 and Bax and down-regulation of anti-apoptotic protein Bcl-2 in U87 cells. We also observed elevation of PARP and Caspase-3 cleavage, which is consistent with increased incidence of apoptosis. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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