External factors
| Category: |
Chemical compounds |
| Phenotype: |
Cerebrovascular aging |
| Experiment: |
SA-β-gal activity assay//Western blot |
| Description: |
Two different concentrations of AngII (10-7 M and 10-6 M) incubation for 5 days caused significant positive β-gal staining (blue) in cultured hBVSMCs,Two concentrations of AngII incubation also triggered NBS1 protein(molecular marker of senescence)level in hBVSMCs. |
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Regulatory relationship
| R-EF-Target gene:
|
Downregulation |
| Official symbol(s): |
TERF2 |
| Target gene experiment: |
Western blot |
| Target gene description: |
AngII caused significant downregulation of TRF2. |
| Regulatory pathway:
|
p53//Siah-1 |
| R-EF-Pathway:
|
Upregulation//Upregulation |
| Official symbol(s): |
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TP53//SIAH1
|
| Pathway experiment: |
Western blot |
| Pathway description: |
AngII treatment for 5 days enhanced total p53 protein expression by ~4.5 folds in hBVSMCs as well as the phospho-p53 expression.FGF21 inhibited Siah-1 expression induced by AngII. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.