External factors
| External factors: |
lipopolysaccharide |
| Category: |
Chemical compounds |
| Tissue type: |
Human impacted third molar |
| Experiment: |
Flow cytometry//SA-β-gal activity assay//Cell morphological analysis |
| Description: |
A senescence-like morphology was observed after repeated stimulation with LPS for 3 or 6 times. The morphology of DPSCs was characterized by a flat shape and increased size.Flow cytometry showed that more DPSCs were restricted to the G1 phase harvested from DPSCs treated with LPS 3 times and 6 times (3 times: 62.46 %±4.6 %, 6 times:66.80 %±4.8 %) than in the DPSCs from the control group or DPSCs treated with LPS only once (control: 45.09 %±3.6 %,once: 46.23 %±3.9 %;).After treatment with LPS, the number of SA-β-gal–positive cells clearly increased in LPS-treated DPSCs, with the strongest staining being seen in DPSCs receiving LPS stimulation 6 times. |
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Regulatory relationship
| Target gene:
|
γ-H2A.X//P16 |
| R-EF-Target gene:
|
Upregulation//Upregulation |
| Official symbol(s): |
H2AX//P16 |
| Target gene experiment: |
Western blot//RT-PCR//qRT-PCR |
| Target gene description: |
The result suggested that the stimulation of LPS 3 or 6 times markedly increased γ-H2A.X protein expression. Analysis by reverse transcription plus PCR (RT-PCR) showed that levels of γ-H2A.X were up-regulated after exposure to LPS 3 or 6 times.After stimulation with LPS, the expression level of p16INK4A was up-regulated in a manner dependent on the number of repeated treatments. |
| R-EF-Pathway:
|
Upregulation |
| Official symbol(s): |
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TLR4
|
| Pathway experiment: |
Western blot |
| Pathway description: |
After stimulation with LPS, the expression level of TLR4 was upregulated in a manner dependent on the number of repeated treatments . |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.