| Gene name: | METTL3 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | hMSCs |
| Gene ID: | 56339 |
| Category: | protein coding |
| Phenotype: | Progeroid syndromes |
| Experimental category: | HL |
| PMID: | 33035345 |
| Experiment: | SA-β-gal activity assay//Clonal expansion assay |
| Description: | Furthermore, METTL3-deficient hMSCs acquired premature aging phenotypes, as evidenced by decreased proliferative capacity and increased percentage of SA-β-Gal-positive cells. Moreover, enhanced cell proliferative capacity and reduced SA-β-Gal-positive cells were observed in HGPS and WS hMSCs upon METTL3 overexpression. |
| Target gene: | MIS12 |
| Official symbol(s): | MIS12 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Methylation |
| Target gene experiment: | qRT-PCR//Western blot |
| Target gene description: | Among these transcripts, we noticed that m6A modifications in MIS12, a key regulator of cell proliferation, were markedly reduced in both prematurely senescent and METTL3-deficient hMSCs, as validated by MeRIP-qPCR analysis. We also detected a significant decrease in MIS12 expression at both the mRNA and protein levels in HGPS and WS hMSCs as well as in METTL3-deficient hMSCs. Among these transcripts, we noticed that m6A modifications in MIS12, a key regulator of cell proliferation, were markedly reduced in both prematurely senescent and METTL3-deficient hMSCs, as validated by MeRIP-qPCR analysis. We also detected a significant decrease in MIS12 expression at both the mRNA and protein levels in HGPS and WS hMSCs as well as in METTL3-deficient hMSCs. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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