| Gene name: | PDK1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | NHDF |
| Experiment: | SA-β-gal activity assay//qRT-PCR//EdU assay |
| Description: | Either PDK1 inhibition or dual inhibition of mTOR and NF-κB reduced the SA-β-gal–positive cell population from >80% to <40%, whereas mTOR inhibition alone did not significantly alter the percent of SA-β-gal–positive cells. We found that the percent of proliferating cells increased after either PDK1 inhibition or dual inhibition of mTOR and NF-κB, but not after only mTOR inhibition. Senescent cells showed a significantly increased expression of these SASP-associated genes. Either exposure to the NF-κB inhibitor JSH23 or the PDK1 inhibitor BX795 reduced the expression of these genes. |
| Target gene: | MTOR//NF-κB |
| Official symbol(s): | MTOR//NFKB1 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR//EdU assay |
| Target gene description: | We found that the percent of proliferating cells increased after either PDK1 inhibition or dual inhibition of mTOR and NF-κB, but not after only mTOR inhibition. Senescent cells showed a significantly increased expression of these SASP-associated genes. Either exposure to the NF-κB inhibitor JSH23 or the PDK1 inhibitor BX795 reduced the expression of these genes. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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