| Gene name: | UNC5B |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HUVEC |
| Experiment: | qRT-PCR//EdU assay//CCK-8 assay//SA-β-gal activity assay |
| Description: | The proportion of senescent cells was lower among UNC5B-silenced cells than among the control. The mRNA expression of the SASP genes, i.e., IL-1α, IL-1β, IL-8, MCP-1, and ICAM-1, was significantly attenuated by inhibition of UNC5B. Further, the proliferation of control and UNC5B-silenced cells was assessed. As a result, UNC5B-silenced cells proliferated at a faster rate than the control cells based on the growth curve analysis and Edu incorporation assay. These results reveal that the downregulation of UNC5B ameliorated the senescence-associated phenotype. UNC5B-overexpressing cells displayed an increase in the proportion of SA-β-gal-positive cells than control cells. The mRNA expression of the SASP genes, i.e., IL-1α, IL-1β, IL-8, MCP-1, and ICAM-1, was also significantly upregulated in UNC5B-overexpressing cells compared to that of the controls. Moreover, overexpression of UNC5B reduced total cell numbers according to growth curve analysis and inhibited cell proliferation potential as indicated by fewer Edu-positive cells. |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | Activation |
| Pathway experiment: | Western blot//EdU assay//CCK-8 assay |
| Pathway description: | UNC5B-silencing cells showed downregulated protein expression levels of P53 and P21. For further clarification on the relationship between the P53 pathway and senescence induced by UNC5B, this work used PFTα, a P53-specific inhibitor. The presence of PFTα inhibited the upregulation of P53 induced by UNC5B overexpression and partially suppressed the expression of P21. Additionally, the presence of PFTα reversed the decrease in the percentage of Edu-positive cells induced by UNC5B overexpression. |
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