| Gene name: | SIRT1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | NP |
| Experiment: | SA-β-gal activity assay//Western blot//ROS staining//JC-1 probe staining |
| Description: | The degree of senescence of the NP cells subjected to 0% or 20% (high-magnitude) compressive deformation was analyzed using SA-β-Gal staining (200×). The SIRT1, PGC1α and senescence-related protein (AMPK, P53, P21 and P16) levels were analyzed by Western blotting. After high-compression or high-compression plus Ad-SIRT1 treatment, the ROS level in NP cells was observed and measured by fluorescence microscopy (200×) and flow cytometry following DCFH-DA fluorescent probe staining. JC-1 probe staining (Beyotime, China) was utilized to detect the mitochondrial membrane potential of NP cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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