| Gene name: | SIRT6 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | LFH cells |
| Gene ID: | 51548 |
| Category: | protein coding |
| Phenotype: | Lumbar spinal stenosis |
| Experimental category: | HL |
| PMID: | 33568575 |
| Experiment: | SA-β-gal activity assay//qRT-PCR |
| Description: | SIRT6 overexpression significantly reduced the frequency of SA-β-gal-positive LFH cells to 11.8%. Trends in the expression of senescence- related genes including p16INK4a, MMP3, and long interspersed nuclear element 1 were consistent with frequencies of SA-β-gal-positive cells, with the exception of L1, which was inhibited in pSIRT6 and sh- hTERT co-transduced cells, while the same was not true for p16 or MMP3. |
| Target gene: | HTERT |
| Official symbol(s): | HTERT |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | SA-β-gal activity assay//qRT-PCR |
| Target gene description: | SIRT6 overexpression significantly reduced the frequency of SA-β-gal-positive LFH cells to 11.8%, whereas hTERT knockdown increased this frequency to 28.4%. Importantly, hTERT knockdown in SIRT6-overexpressing cells reversed the beneficial impact of SIRT6 overexpression and increased the frequency of SA-β-Gal positive LFH cells to 26.2%. Trends in the expression of senescence- related genes including p16INK4a, MMP3, and long interspersed nuclear element 1 were consistent with frequencies of SA-β-gal-positive cells, with the exception of L1, which was inhibited in pSIRT6 and sh- hTERT co-transduced cells, while the same was not true for p16 or MMP3. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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