| Gene name: | MIR340 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HT22 |
| Gene ID: | 442908 |
| Category: | ncRNA |
| Phenotype: | Alzheimer's disease |
| Experimental category: | L |
| PMID: | 33624913 |
| Experiment: | SA-β-gal activity assay//MTT assay |
| Description: | Compared with untreated HT22 cells, the viability of Aβ42 induced cell viability was decreased, the apoptotic rate was increased, and intracellular β galactose deposition was increased, which suggested accelerated cellular senescence, but these signs were alle- viated after POT1 downregulation.（删除？）Compared with cells in the Aβ42 group, cell viability was increased, apoptosis rate was decreased, and intracellular β galactose deposition was reduced after overexpression of miR-340-5p. |
| Target gene: | POT1 |
| Official symbol(s): | POT1 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Dual-Luciferase reporter assay//qRT‐PCR//SA-β-gal activity assay |
| Target gene description: | A dual luciferase reporter vector was con- structed to validate the targeted binding relationship between miR 340 5p and POT1. MiR-340-5p was overexpressed using agomiRNA in Aβ42 induced HT22 cell, and intracellular POT1 level was decreased after overexpression of miR-340-5p. Compared with cells in the Aβ42 group, cell viability was increased, apoptosis rate was decreased, and intracellular β galactose deposition was reduced after overexpression of miR-340-5p. TRF analysis showed that cel- lular telomere length was significantly increased. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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