| Gene name: | MIR99B |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Chondrocyte |
| Experiment: | Western blot |
| Description: | We then explored the role of miR-99b-5p in OA development. Increased MMP13 and decreased COL2 were detected in mimics-treated chondrocytes, accompanied by enhanced senescence-related hallmarks P16, P21, and P53. |
| Target gene: | MFG-E8 |
| Official symbol(s): | MFG-E8 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | We found inhibited expression of MFG-E8 in cells treated with miR-99b-5p mimics and upregulated expression in cells treated with miR-99b-5p inhibitor. |
| Regulatory pathway: | P65 NF-ΚB |
| R-AG-Pathway: | Activation |
| Pathway experiment: | Immunofluorescence//Western blot |
| Pathway description: | We found that MFG-E8 treatment rescued the phosphorylation of p65 NF-κB signaling, while neutralizing MFG-E8 significantly enhanced p65 phosphorylation both in cartilage and in synovium post OA surgery. In vitro studies in murine primary chondrocytes and RAW264.7 cells con- firmed the suppressive effect of MFG-E8 in p65 NF-κB signaling. |
Annotation:
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