| Gene name: | MIR31 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MDA-MB-231,MCF-7,MRC-5 |
| Experiment: | SA-β-gal activity assay//EdU assay//Immunostaining |
| Description: | The data showed that indeed, miR-31 overexpression increased SA-β-gal positive cells and decreased EdU positive cells indicating that miR-31 is a potent inducer of cell senescence. The role of miR-31 in senescence was further confirmed by examining γH2AX foci formation, which is another marker of cell senescence. The data indicated that miR-31 overexpression leads to an increase in γH2AX foci formation in MRC5 cells.Induction of senescence by miR-31 overexpression was also confirmed in MDA-MB-231 and MCF7 cells. |
| Target gene: | BMI1 |
| Official symbol(s): | BMI1 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | We found that miR-31 overexpression leads to repression of BMI1 and its inhibitor up-regulates BMI1.miR-31 overexpression also led to significant down-regulation of BMI1. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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