| Gene name: | MIR34A |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | Bone |
| Cell name: | Chondrocyte |
| Experiment: | SA-β-gal activity assay//Flow cytometry//CCK-8 assay |
| Description: | Cells characterized by positive SA-β-gal were enhanced in chondrocytes that underwent transfection with the miR-34a mimic.Compared to the TRAIL alone treatment group, the inhibitor of miR-34a suppressed the number of apoptotic cells.The miR-34a mimic transfection remarkably suppressed the growth of these cells. |
| Target gene: | DLL1 |
| Official symbol(s): | DLL1 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Luciferase reporter assay//Western blot |
| Target gene description: | In order to explore the interaction between miR-34a and the DLL1 3′-UTR, the miR-34a binding region was mutated in the DLL1 3′-UTR. Subsequent to luciferase vector co-transfection with DLL1 3′-UTR as well as the miR-34a mimic, the miR-34a mimic was discovered to be unable to inhibit expression of luciferase. The findings indicated that transcription, secretion, and translation of DLL1 were reversely modulated through miR-34a. |
| Regulatory pathway: | PI3K-AKT |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | PIK3CA-AKT1 |
| Pathway experiment: | Western blot |
| Pathway description: | He findings revealed that the level of total AKT and p-AKT declined in the groups that underwent miR-34a transfection. |
Annotation:
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