| Gene name: | MIR380 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Mammary Gland |
| Cell name: | Mammary epithelial cell |
| Experiment: | SA-β-gal activity assay |
| Description: | Tumor cells expressing activated HRAS plus control (empty vector or scrambled control) stained positive for senescence-associated β-galactosidase. In contrast, tumors expressing HRAS plus miR-380 or shRNA p53 did not express senescence markers . |
| Target gene: | P21 |
| Official symbol(s): | P21 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Immunohistochemical staining//qRT-PCR |
| Target gene description: | p21waf1 was increased in wild-type mouse mammary epithelial cells and tumor cells expressing activated HRAS plus control. In contrast, p21waf1 expression was lower in tumor cells that co-express HRAS and miR-380 or shRNA p53. |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | Expression of miR-380-5p resulted in a significant ~40% decrease in basal p53 protein levels. UV irradiation led to a dose-dependent increase in p53 protein expression that was suppressed by the expression of miR-380-5p . |
Annotation:
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