| Gene name: | MIR150 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Normal NK/T-cell Lymphoma cell line cell,HANK-1,MOTN-1,SNK-6 |
| Gene ID: | 406942 |
| Category: | ncRNA |
| Phenotype: | Malignant lymphoma |
| Experimental category: | L |
| PMID: | 21502955 |
| Experiment: | SA-β-gal activity assay//TRAP assay//Southern blot analysis |
| Description: | In addition, these cells stained positively for Ki-67 and senescence-associated beta-gal on day 21 after GFP selection and miR-150 transductants had a ‘fried egg’-like appearance, which is caused by senescence and is not seen in normal NK/T-cell lymphoma cells.Telomerase activity in miR-150 transductants (on day 21 after GFP selection) was markedly lower than in cells transduced with empty vector.We observed a shortening of telomeric DNA in the cells expressing miR-150 during continuous culture. Indeed, the telemetric restriction fragments of miR-150 transductants grew shorter with every successive passage. |
| Regulatory pathway: | PI3K-AKT |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | PIK3CA-AKT1 |
| Pathway experiment: | Luciferase reporter assay//Western blot |
| Pathway description: | Upon insertion of the wild-type 30-UTR of DKC1, PIK3AP1, AKT3 or AKT2 into the reporter, we observed significant reductions in luciferase activity with DKC1, PIK3AP1 and AKT2, but not in AKT3 (data not shown), as compared with cells transduced with empty vector(control vector), suggesting that DKC1, PIK3AP1 (data not shown) and AKT2 have potential to function as direct targets of miR-150 .We therefore carried out a western analysis of pAKTser473/4expression and found it to be markedly reduced in the cells tested. We also detected upregulation of Bim (in MOTN-1, HANK-1 and SNK-6) and p53 (in SNK-6 and HANK-1). |
Annotation:
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