| Gene name: | MIR127 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | WI-38,IMR-90 |
| Experiment: | SA-β-gal activity assay//Western blot//PI staining//Cell counting |
| Description: | We observed that induced miR-127 expression caused a remarkable inhibition of cell proliferation and increased senescence-like phenotypes with positive staining of senescence-associated-β-galactosidase (SA-β-gal) in both WI-38 and IMR-90 cells.the senescence-like phenotype was associated with up-regulation of p53 and p21 and down-regulation of cyclin D1 (a pattern associated with senescence) in both WI-38 and IMR-90 cells.miR-127 overexpression induced cell cycle arrest at G0/G1phase. |
| Target gene: | BCL6 |
| Official symbol(s): | BCL6 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | Over-expression of miR-127 has markedly down-regulated BCL6 expression in both WI-38 and IMR-90 cells. |
| Regulatory pathway: | P53-P21 |
| R-AG-Pathway: | Upregulation |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | Western blot |
| Pathway description: | The depletion of BCL6 inhibited cell proliferation was associated with increased p53 and p21 expression . |
Annotation:
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